![]() ![]() ![]() Ammonium sulphate (AS) was used for antibiotic recovery and it was desalted by dialysis techniques. The antimicrobial produced was stable and active even at 55C. Activity was evaluated by well diffusion assay. There were two most important factors that affect the production of antibiotic including time of incubation and nitrogen concentration and two interactions including the time of incubation/pH and time of incubation/nitrogen concentration. The current experiment was designed to optimize the production of novel thermostable polypeptide by applying the Taguchi statistical approach at various conditions including the time of incubation, temperature, pH, aeration rate, nitrogen, and carbon concentrations. A thermophilic Geobacillus pallidus SAT4 was isolated from hot climate of Sindh Dessert, Pakistan and found it active against Micrococcus luteus ATCC 10240, Staphylococcus aureus ATCC 6538, Bacillus subtills NCTC 10400 and Pseudomonas aeruginosa ATCC 49189. Abstract: Polypeptide antimicrobials used against topical infections are reported to obtain from mesophilic bacterial species. ![]()
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